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Literature summary extracted from

  • Bucurenci, N.; Serina, L.; Zaharia, C.; Landais, S.; Danchin, A.; Barzu, O.
    Mutational analysis of UMP kinase from Escherichia coli (1998), J. Bacteriol., 180, 473-477.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.22 GTP most effective at pH 8 and a high (1 mM) UMP concentration Escherichia coli

Application

EC Number Application Comment Organism
2.7.4.22 drug development potential target for antibacterial drugs Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.22
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.22 D146N 84% of wild-type activity in the pellet of the sonicated bacterial extract Escherichia coli
2.7.4.22 D159N exhibits higher solubility than the wild-type protein at neutral pH, 10% of wild-type activity in the pellet of the sonicated bacterial extract Escherichia coli
2.7.4.22 D168N 97% of wild-type activity in the pellet of the sonicated bacterial extract Escherichia coli
2.7.4.22 D174N loss of two-thirds of its activity after 3 months storage at 4°C in 50 mM Tris-HCl (pH 7.4), 98% of wild-type activity in the pellet of the sonicated bacterial extract Escherichia coli
2.7.4.22 D201N exhibits higher solubility than the wild-type protein at neutral pH, 42% of wild-type activity in the pellet of the sonicated bacterial extract Escherichia coli
2.7.4.22 D77N 84% of wild-type activity in the pellet of the sonicated bacterial extract, insensitive to activation by GTP Escherichia coli
2.7.4.22 R62H 83% of wild-type activity in the pellet of the sonicated bacterial extract, insensitive to activation by GTP Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.22 GTP mutant D201N is inhibited by GTP above 0.2 mM Escherichia coli
2.7.4.22 UTP the inhibitory effect is maximal at pH 8 and a low (0.1 mM) UMP concentration Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.22 0.03
-
 ATP mutant D146N, at pH 6 Escherichia coli
2.7.4.22 0.043
-
 UMP wild-type, at pH 8 Escherichia coli
2.7.4.22 0.048
-
 ATP wild-type, at pH 6 Escherichia coli
2.7.4.22 0.052
-
 UMP mutant D159N, at pH 8 Escherichia coli
2.7.4.22 0.059
-
 UMP mutant D146N, at pH 8 Escherichia coli
2.7.4.22 0.06
-
 UMP mutant D168N, at pH 8 Escherichia coli
2.7.4.22 0.095
-
 ATP mutant D159N, at pH 6 Escherichia coli
2.7.4.22 0.1
-
 ATP mutant D146N, at pH 8 Escherichia coli
2.7.4.22 0.12
-
 ATP mutant D77N, at pH 6 Escherichia coli
2.7.4.22 0.12
-
 ATP wild-type, at pH 8 Escherichia coli
2.7.4.22 0.14
-
 ATP mutant D168N, at pH 6 Escherichia coli
2.7.4.22 0.17
-
 ATP mutant D174N, at pH 6 Escherichia coli
2.7.4.22 0.17
-
 UMP wild-type, at pH 6 Escherichia coli
2.7.4.22 0.2
-
 ATP mutant D201N, at pH 6 Escherichia coli
2.7.4.22 0.22
-
 UMP mutant D201N, at pH 8 Escherichia coli
2.7.4.22 0.24
-
 ATP mutant R62H, at pH 6 Escherichia coli
2.7.4.22 0.27
-
 UMP mutant D159N, at pH 6 Escherichia coli
2.7.4.22 0.29
-
 ATP mutant D159N, at pH 8 Escherichia coli
2.7.4.22 0.31
-
 ATP mutant D174N, at pH 8 Escherichia coli
2.7.4.22 0.31
-
 UMP mutant D174N, at pH 8 Escherichia coli
2.7.4.22 0.32
-
 UMP mutant D168N, at pH 6 Escherichia coli
2.7.4.22 0.32
-
 UMP mutant R62H, at pH 8 Escherichia coli
2.7.4.22 0.42
-
 ATP mutant D168N, at pH 8 Escherichia coli
2.7.4.22 0.45
-
 UMP mutant D146N, at pH 6 Escherichia coli
2.7.4.22 0.46
-
 ATP mutant D201N, at pH 8 Escherichia coli
2.7.4.22 0.47
-
 UMP mutant D77N, at pH 8 Escherichia coli
2.7.4.22 0.48
-
 ATP mutant D77N, at pH 8 Escherichia coli
2.7.4.22 0.86
-
 UMP mutant D201N, at pH 6 Escherichia coli
2.7.4.22 1.26
-
 UMP mutant R62H, at pH 6 Escherichia coli
2.7.4.22 1.78
-
 UMP mutant D77N, at pH 6 Escherichia coli
2.7.4.22 3
-
 ATP mutant R62H, at pH 8 Escherichia coli
2.7.4.22 3.7
-
 UMP mutant D174N, at pH 6 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.22 150000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.22 ATP + UMP Escherichia coli
-
 ADP + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.22 Escherichia coli P0A7E9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.22
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.22 0.15
-
 mutant D146N, at pH 6 Escherichia coli
2.7.4.22 0.32
-
 mutant D146N, at pH 8 Escherichia coli
2.7.4.22 1.04
-
 mutant D201N, at pH 6 Escherichia coli
2.7.4.22 1.5
-
 mutant R62H, at pH 6 Escherichia coli
2.7.4.22 1.9
-
 mutant D77N, at pH 6 Escherichia coli
2.7.4.22 4.4
-
 mutant R62H, at pH 8 Escherichia coli
2.7.4.22 8.2
-
 mutant D201N, at pH 8 Escherichia coli
2.7.4.22 8.2
-
 mutant D77N, at pH 8 Escherichia coli
2.7.4.22 32
-
 mutant D174N, at pH 6 Escherichia coli
2.7.4.22 45
-
 mutant D174N, at pH 8 Escherichia coli
2.7.4.22 63
-
 mutant D168N, at pH 6 Escherichia coli
2.7.4.22 86.6
-
 mutant D168N, at pH 8 Escherichia coli
2.7.4.22 105
-
 wild-type, at pH 6 Escherichia coli
2.7.4.22 128
-
 mutant D159N, at pH 6 Escherichia coli
2.7.4.22 128
-
 wild-type, at pH 8 Escherichia coli
2.7.4.22 153
-
 mutant D159N, at pH 8 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.4.22 4°C, 50 mM Tris-HCl (pH 7.4), several months, no significant loss of activity. An exception is mutant D174N, which loses two-thirds of its activity after 3 months at 4°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.22 ATP + UMP
-
 Escherichia coli ADP + UDP
-
 ?
2.7.4.22 ATP + UMP 30°C, pH 6, 7.4 or 8, 2 mM MgCl2 Escherichia coli ADP + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.22 hexamer alpha6, gel filtration Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.4.22 UMP kinase
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.22 48
-
 Tm for mutant D201N Escherichia coli
2.7.4.22 52
-
 Tm for mutant D77N Escherichia coli
2.7.4.22 59 62 mutants R62H, D146N, D168N and D174N are half inactivated between 59 and 62°C Escherichia coli
2.7.4.22 63
-
 wild-type Tm Escherichia coli
2.7.4.22 73
-
 Tm for mutant D159N Escherichia coli